Journal: Cell Death & Disease

Article Title: Imatinib induces ferroptosis in gastrointestinal stromal tumors by promoting STUB1-mediated GPX4 ubiquitination

doi: 10.1038/s41419-023-06300-2

Figure Lengend Snippet: A GIST-T1 cells were subcutaneously transplanted into the dorsolateral side of nude mice. When the subcutaneous tumor volume reached 50 mm 3 , the mice were randomly divided into four groups (six mice in each group), including the vehicle (DMSO), IM (100 mg/kg), RSL3 (10 mg/kg) or IM combined with RSL3 treatment groups, and then were injected intraperitoneally every there day. All mice were euthanized 18 days later. B Body weight was measured every 3 days until day 18. C The images and quantitative analysis of tumor volume on day 18 after treatment. D – F Western blot analysis of the protein levels of Ki-67, KIT, STUB1, GPX4, and 4-HNE in GIST cell xenograft tumor tissues of mice from vehicle (DMSO), IM, RSL3, and combination treatment groups. n = 3 mice per group. G Immunohistochemical staining representative pictures of hematoxylin-eosin (HE), KIT, STUB1, GPX4, Ki-67, and 4-HNE in GIST cell xenograft tumor tissues of mice from vehicle (DMSO), IM, RSL3, and combination treatment groups. Scale bar, 100 μm. DMSO dimethyl sulfoxide, IM Imatinib, RSL3 Ras-selective lethal small molecule 3, 4-HNE 4-hydroxy-2-nonenal. Significance denoted by: ns not significant, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: RSL3 was dissolved in DMSO, diluted in corn oil (#HY-Y1888, MCE), and then intraperitoneally injected into mice with IM (100 mg/kg) or RSL3 (10 mg/kg) every 3 days.

Techniques: Injection, Western Blot, Immunohistochemical staining, Staining

Journal: eLife

Article Title: Canonical NF-κB signaling maintains corneal epithelial integrity and prevents corneal aging via retinoic acid

doi: 10.7554/eLife.67315

Figure Lengend Snippet:

Article Snippet: For in vivo experiments, RA was dissolved in corn oil while axitinib (Selleck Chemicals) was dissolved in dimethyl sulfoxide, which were reconstituted in PEG300/Tween80/Water mixed solution.

Techniques: Sequencing, SYBR Green Assay, Chromatin Immunoprecipitation, Eye Drops, Software